Abstract

𝘓𝘪𝘵𝘴𝘦𝘢 𝘮𝘰𝘯𝘰𝘱𝘦𝘵𝘢𝘭𝘢 (Roxb.) Pers. is a plant of significant ethnobotanical importance, and in this study, we conducted a comprehensive investigation into its potential bioactive properties. Different fractions of the ethyl acetate (EtOAc) crude extract of the plant leaves were evaluated for antioxidant, cytotoxicity, thrombolytic, and anti-inflammatory activities, 𝘐𝘯 𝘷𝘪𝘵𝘳𝘰. The hexane-soluble fraction (LMH) exhibited notable antioxidant activity with IC50 value of 5.44 μg/ml and cytotoxicity with LC50 value of 1.10 μg/ml in DPPH radical scavenging assay and Brine shrimp lethality bioassay, respectively. Moreover, Dichloromethane soluble fraction (LMD) exhibited the highest thrombolytic activity, achieving an impressive rate of 31 %, compared to the standard Streptokinase (45 %). Additionally, LMD demonstrated a significant inhibition of 36 % of hemolysis, while LMH exhibited a slightly lower inhibition rate of 33 %, while the standard drug acetyl salicylic acid achieved a 47 % inhibition of hemolysis, suggesting their relevance in the context of inflammation-related disorders. These results underscore the multifaceted pharmacological properties of 𝘓𝘪𝘵𝘴𝘦𝘢 𝘮𝘰𝘯𝘰𝘱𝘦𝘵𝘢𝘭𝘢 and provide a solid foundation for further research, including the isolation and characterization of bioactive compounds, elucidation of underlying mechanisms, and potential applications in drug development and healthcare.