Abstract

The aim of this study was to evaluate the bioactive compounds identification and determine the potential antioxidant and alpha(α)-amylase inhibitory activities of Wheat Bran (WB) extract. Phytochemical analysis was done by high performance liquid chromatography (HPLC) and gas chromatography (GC) in ethanol and n-hexane extract. Antioxidant potential was determined by nitric oxide scavenging and DPPH free radical scavenging assays. HPLC analysis showed the presence of dihydroxybenzoic acid, catechin hydrate, (-) epicatechin, caffeic acid, rutin hydrate, p-coumaric acid, trans-ferulic acid, rosmarinic acid, quercetin; and kaempferol. GC analysis showed a good number of compounds present in the WB extracts. The extracts showed considerable free radicles scavenging activity in both nitric oxide scavenging and DPPH free radical scavenging assays. IC50 of WB ethanol extract in DPPH scavenging assay was found 39.00 µg/mL and 14.57 µg/mL for ascorbic acid. In the in vitro α-amaylase inhibitory activity assay, the IC50 value of WB ethanol extract was 61.97 µg/mL, whereas the IC50 value for standard drug acarbose was 35.80 µg/mL. This investigation revealed that, WB extract is a potential source of bioactive compounds which can be used as alternative supplements for natural antioxidants.